Circular Dichroism of Ribonuclease A, Ribonuclease S, and Some Fragments
نویسندگان
چکیده
منابع مشابه
Optical activity of cystine-containing proteins. II. Circular dichroism spectra of pancreatic ribonuclease A, ribonuclease S, and ribonuclease S-protein.
The circular dichroism spectra of pancreatic ribonuclease A, RNase S, and RNase S-protein have been measured in the wave length interval 198 to 300 mp under various conditions. The circular dichroism spectra of RNase A and S are very similar, possibly identical, over this entire wave length interval at neutral pH. That of RNase S-protein is substantially different. It is shown that the portion ...
متن کاملThermal effects on the circular dichroism spectra of ribonuclease A and of ribonuclease S-protein.
The thermal denaturation of ribonuclease A and of S-protein in neutral aqueous solutions has been studied by means of their circular dichroism spectra. For RNase A, the positive circular dichroism extremum near 241 rnp shows a biphasic dependence upon temperature, a linear change between 15” and 50” being followed by a sharp one above 52”. The negative circular dichroism extremum at 277 rnp exh...
متن کاملKinetic circular dichroism shows that the S-peptide alpha-helix of ribonuclease S unfolds fast and refolds slowly.
It is shown that circular dichroism (CD) can distinguish between the S-peptide and the S-protein fragments of RNase S at 225 nm and 235 nm. The conformational source for the strong CD at 225 nm is the S-peptide alpha-helix. The structural assignment of the CD at 235 nm is not clear but it is shown to be largely due to the S-protein moiety. This situation is utilized to monitor the kinetics of p...
متن کاملStudies on Ribonuclease S
Specific regions of the polypeptide sequence of pancreatic ribonuclease have been altered by chemical modification and by limited proteolytic digestion in attempts to implicate specific covalent portions of the molecule in the structure and stabilization of the active center. Digestion of the native molecule with trypsin at elevated temperatures has been shown to remove portions of the chain wi...
متن کاملCrystalline semisynthetic ribonuclease-S'.
Crystals of solid phase-derived semisynthetic ribonuclease-S' were prepared and compared with those for native ribonuclease-S' and -S. The semisynthetic species used was the noncovalent complex of synthetic fragment-(1-20), corresponding to residues 1 through 20 of bovine pancreatic ribonuclease-A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, EC 3.1.4.22), and native ribonuclease-S-(21-...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99347-6